1. Rapid detection of antibiotic resistance by MALDI-TOF mass spectrometry using a novel direct-on-target microdroplet growth assay 
E.A. Idelevich, K. Sparbier, M. Kostrzewa, K. Becker 
Clinical Microbiology and Infection 
Volume 24, Issue 7, Pages (July 2018)
DOI: /j.cmi
Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

2. Fig. 1
The experimental setup. (a) Detail of a MALDI-TOF MS target with microdroplets before incubation. Rows A, B, C, D and E contain microdroplets with total volumes of 2, 4, 6, 8 and 10 μL, respectively. Columns 1 to 3, susceptible Klebsiella pneumoniae isolate with meropenem; columns 4 to 6, growth control of susceptible K. pneumoniae isolate without antibiotic; columns 7 to 9, resistant K pneumoniae isolate with meropenem; columns 10 to 12, growth control of resistant K. pneumoniae isolate without antibiotic. (b) MALDI-TOF MS target with microdroplets in a ‘humidity chamber’. (c) Separation of nutrient broth from microbial cells by capillary effects using a tissue wipe as an absorptive material.
Clinical Microbiology and Infection  , DOI: ( /j.cmi )
Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

3. Fig. 2
Evaluation of the MALDI-TOF MS data by a simple algorithm. (a) Meropenem-resistant isolate, MALDI Biotyper finding: Klebsiella pneumoniae. (b) Meropenem-susceptible isolate, MALDI Biotyper finding: No identification. Successful species identification by means of MALDI Biotyper software (score ≥1.7) was defined as a successful growth detection. Successful growth detection (score ≥1.7) of a sample with antibiotic defined a resistant isolate, while no growth detection (score <1.7) in the presence of antibiotic defined susceptible isolate.
Clinical Microbiology and Infection  , DOI: ( /j.cmi )
Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions