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Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy ATG16L1−/− cells reconstituted with ATG16L1WT or ATG16L1LE.

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Presentation on theme: "Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy ATG16L1−/− cells reconstituted with ATG16L1WT or ATG16L1LE."— Presentation transcript:

1 Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy
ATG16L1−/− cells reconstituted with ATG16L1WT or ATG16L1LE were cultured in full growth media prior to immunofluorescence analyses using antibodies against WIPI2 (green) or ATG16L1 (red). Scale bar: 9 μm. Cells as in (A) with the addition of 3′MA treatment for 30 min. Scale bar: 9 μm. Quantification of percentage of cells positive for ATG16L1 puncta in (A) and (B). Quantifications depict means and error bars (SEM) from at least three independent experiments. ns P > 0.05, ***P ≤ 0.001 (pairwise unpaired Student's t‐test). Ferroptosis assay in ATG16L1−/− cells stably expressing F‐S‐tagged ATG16L1WT, ATG16L1LD or ATG16L1LE. Cells were cultured in amino acid free media (AA starve) in the presence of 10% FBS or 10% dialysed FBS (diFBS) for 24 h. Representative PI staining and phase contrast images are shown (relevant to Fig G). Scale bar: 30 μm. Western blot analyses of ATG16L1−/− cells stably expressing F‐S‐tagged ATG16L1WT, ATG16L1LD or ATG16L1LE using the indicated antibodies (relevant to D and Fig G). IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 38, Issue: 9, First published: 01 April 2019, DOI: ( /embj )


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