1. A, Representative voltage-clamp recordings from a CRF1+ neuron during superfusion of GBZ (100 μm), followed by PTX (100 μm, top trace) and during superfusion of PTX followed by GBZ (bottom trace).
A, Representative voltage-clamp recordings from a CRF1+ neuron during superfusion of GBZ (100 μm), followed by PTX (100 μm, top trace) and during superfusion of PTX followed by GBZ (bottom trace). Dashed lines indicate average holding current. B, Summary of the tonic current in CRF1+ neurons revealed by superfusion of GBZ (n = 10) and PTX (n = 9) and occlusion of tonic current by previous superfusion of either GBZ (n = 5) or PTX (n = 5); *p < 0.05 by one-sample t test, #p < 0.05 by unpaired t test. C, Representative voltage-clamp recordings from a CRF1− neuron during superfusion of GBZ (100 μm, top trace) and during superfusion of nipecotic acid (NIP, 1 mm) followed by GBZ (bottom trace). Dashed lines indicate average holding current. D, Summary of the tonic current in CRF1− neurons revealed by superfusion of GBZ alone (n = 7) and by superfusion of GBZ in the presence of NIP (n = 6). *p < 0.05 by one-sample t test, #p < 0.05 by unpaired t test.
Melissa A. Herman et al. J. Neurosci. 2013;33:
©2013 by Society for Neuroscience