1. Fertility Center
Put on ice twice: a problem? Comparison of trophectoderm biopsy (Tebx) with preimplantation genetic screening (pgs) in cycles usiNg previously frozen vs. fresh autologous oocytes
Nicole Noyes, Hsiao-Ling Lee, Sarah Druckenmiller,
Patty Labella, Esmeralda Ampeloguio, James Grifo

2. OBJECTIVE
Use of oocyte cryopreservation and TEBX with PGS has rapidly increased over the past decade
Together, they allow women to have children when ready and limit embryo transfer to a single euploid blastocyst
Aim: to assess whether adding TEBX with PGS to oocyte cryopreservation impacted outcomes
Retrospective cohort study
Reviewed all autologous oocyte cryopreservation and fresh cycles using TEBX with PGS at our large university-based fertility center
Fertility Center

3. OOCYTE CRYOPRESERVATION
RESULTS
OOCYTE CRYOPRESERVATION
(n = 116 cycles)
FRESH
(n = 1446 cycles)
Median age (y)
39 + 2
No. oocytes survived & assessed as MII at thaw
1004 (78%)
Cycles with > 1 blastocyst suitable for TEBX
93/116 (80%)
(mean 4 / cycle)*
1243/1446 (86%)
(mean 5 / cycle)*
TEBX cycles with > 1 euploid blastocyst
57/93 (61%)
753/1243 (61%)
Embryo implantation rate
30/51 (59%)
671/963 (70%)
Ongoing + delivered rate / transfer
27/50 (54%)
566/941 (60%)
Fertility Center

4. CONCLUSIONS
Oocyte cryopreservation was associated with fewer blastocysts suitable for TEBX, but not a lower proportion of cycles with at least one biopsiable blastocyst
Euploidy rate per biopsied blastocyst as well as implantation and ongoing + delivered rate per transferred euploid blastocyst were similar whether starting with frozen or fresh oocytes
Adding TEBX to oocyte cryopreservation appears potentially beneficial in affording single embryo transfer and thus singleton live birth
Fertility Center