1. Activation of membrane-associated Akt is associated with loss of apoptosis in TgAPT121;Pten+/− prostates.
Activation of membrane-associated Akt is associated with loss of apoptosis in TgAPT121;Pten+/− prostates. A, p-Akt is not detected in wild-type prostate (a; 57 weeks), but cytoplasmic p-Akt is consistently detected in TgAPT121 prostate (b; 57 weeks), with no membrane localization detectable up to 90 weeks of age. In Pten+/− mice at ∼1 year (c), focal prostate abnormalities are positive for membrane p-Akt, whereas cytoplasmic p-Akt is present in cells of surrounding glands. B and C, TgAPT121;Pten+/− prostate lesions (B, a-d) with Pten expression show no membrane localization of p-Akt (B, a) and apoptosis is present by TUNEL (B, b). On focal adenocarcinoma progression with loss of Pten expression and induction of membrane-bound p-Akt (B, c), apoptosis is low (B, d, and C).
Reginald Hill et al. Cancer Res 2005;65:
©2005 by American Association for Cancer Research