1. A, Northern blot analysis of DRCTNNB1A.
A, Northern blot analysis of DRCTNNB1A. Left, molecular size (kb) of the transcript. B, chromosomal mapping of the DRCTNNB1A gene by FISH. Metaphase chromosomes were stained with propidium iodide to show twin-spot signals (arrows). C, G-band pattern of the same metaphase chromosomes, delineated through an UV-2A filter (Nikon, Tokyo, Japan), indicates that the DRCTNNB1A gene hybridized specifically to chromosomal band 7p15.3. D, Western blot analysis of extracts from COS7 cells transfected with the plasmid containing DRCTNNB1A/HA. E, cytoplasmic localization of DRCTNNB1A. Cells were stained with anti-HA antibodies and 4′,6-diamidino-2-phenylindole.
Teru Kawasoe et al. Cancer Res 2000;60:
©2000 by American Association for Cancer Research