1. Eight weeks after primary i. n
Eight weeks after primary i.n. immunization with live RSV, iRSV, or medium, five adult BALB/c mice per group per time point were challenged i.n. with 20 μl containing 4 × 105 PFU of RSV. (A) Inductive tissues (NALT, CLN, and BLN) (A) and effector tissues (SM, tracheal lamina propria, and lung) (B) were isolated and cultured.
Eight weeks after primary i.n. immunization with live RSV, iRSV, or medium, five adult BALB/c mice per group per time point were challenged i.n. with 20 μl containing 4 × 105 PFU of RSV. (A) Inductive tissues (NALT, CLN, and BLN) (A) and effector tissues (SM, tracheal lamina propria, and lung) (B) were isolated and cultured. The quantities of RSV-specific IgA produced were determined by ELISA 0, 3, 6, and 9 days after challenge. Data represent the arithmetic mean and standard error of the mean of the percentage of RSV-specific IgA compared to the total IgA produced. *, P < 0.05 compared to the percentage of RSV-specific IgA in samples from control mice; all other differences were not statistically significant (P > 0.05).
Janine Valosky et al. Clin. Diagn. Lab. Immunol. 2005; doi: /CDLI