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Fig. 2. Overexpression of miR cluster in the developing heart results in increased cardiomyocyte proliferation and cardiomegaly. Overexpression of miR cluster in the developing heart results in increased cardiomyocyte proliferation and cardiomegaly. (A) At E18.5, Nkx2.5cre:R26R-miR Tg/+ mutants have an enlarged heart with thickened ventricular myocardium and ventricular septal defects compared to Nkx2.5cre controls. (B) Cardiomegaly and increased cardiomyocyte proliferation in Nkx2.5cre:R26R-miR Tg/+ mutant hearts at P20. (A and B) Immunostainings for PH3 and α-actinin and wheat germ agglutinin (WGA) show the number of mitotic cardiomyocytes. Scale bars, 100 μm. High-magnification reveals PH3+ cardiomyocytes. LA, left atrium. Data are means ± SEM (n = 3). P values determined by Student’s t test. Ying Tian et al., Sci Transl Med 2015;7:279ra38 Copyright © 2015, American Association for the Advancement of Science
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