1. DNA demethylation reactivates GSN expression in gastric cancer cells.
DNA demethylation reactivates GSN expression in gastric cancer cells. Six gastric cancer cell lines were treated with 5-AZA for 24 hours. A, Methylation status of the GSN promoter was evaluated by MSP using unmethylated (U) and methylated (M) primers. B, Methylation levels of four CG units in CpG islands in the GSN promoter were analyzed by pyrosequencing. *, P < 0.05; **, P < 0.01; ***, P < versus controls, two-way ANOVA. C, GSN protein expression was detected by Western blotting analysis. β-actin was used as a loading control. D,GSN mRNA expression was analyzed by qPCR. Data were normalized relative to the untreated control. *, P < 0.05; **, P < 0.01 versus controls, two-way ANOVA.
Hao-Chen Wang et al. Cancer Immunol Res 2017;5:
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