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T cell effector cytokines and staphylococcal products regulate CCL18.

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Presentation on theme: "T cell effector cytokines and staphylococcal products regulate CCL18."— Presentation transcript:

1 T cell effector cytokines and staphylococcal products regulate CCL18.
T cell effector cytokines and staphylococcal products regulate CCL18. Quantitative real-time PCR and ELISA analyses of CCL18 expression in cellular constituents of the skin. a, Analysis of CCL18 in cDNA obtained from cultured human primary keratinocytes, dermal fibroblasts, dermal microvascular endothelial cells treated with medium alone, or with TNF-α/IL-1β, IFN-γ, or IL-4 for 18 h. Values are expressed in femtograms of the target gene in 25 ng of total cDNA. Representative data from single donors. b and c, Analysis of CCL18 mRNA in monocytes, or monocyte-derived dendritic cell subsets and Langerhans cells 6 and 24 h after stimulation with LPS, LTA, PGN, mannan, SEB, and CD40L. Values are expressed in picograms of the target gene in 25 ng of total cDNA. d, ELISA analysis of CCL18 in supernatants obtained from cultured monocytes, DCs, and Langerhans cells treated with medium alone or with LPS, LTA, PGN, mannan, SEB, and CD40L. Values are expressed in nanograms of CCL18 protein produced by 106 cells. Andor Pivarcsi et al. J Immunol 2004;173: Copyright © 2004 by The American Association of Immunologists

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