1. Induction of apoptosis in mismatch repair-deficient tumor cell lines after microinjection of phMSH2 and phMLH1 cDNA expression constructs.
Induction of apoptosis in mismatch repair-deficient tumor cell lines after microinjection of phMSH2 and phMLH1 cDNA expression constructs. The indicated tumor cell lines in A, C, and E were coinjected with the pGFP expression constructs and a mismatch repair gene cDNA construct (phMSH2 for SK-UT-1, phMLH1 for HCT116, and phPMS2 for HEC-1-A). In B, D, and F, the indicated tumor cell lines were microinjected with the empty expression vector (pcDNA3) and the pGFP expression construct. Injected cells were followed by GFP expression. Arrows indicate representative apoptotic cells injected with phMSH2 or phMLH1 found at 24 h postinjection.
Hong Zhang et al. Cancer Res 1999;59:
©1999 by American Association for Cancer Research