1. Neutrophils increase UMSCC47 invasion.
Neutrophils increase UMSCC47 invasion. A, experimental design. The results are calculated on the basis of the area (μm2) occupied by cancer cells that invaded through the Matrigel (seen under the membrane). B, representative images of the underside of the Transwell membrane. C, 50,000 UMSCC47 cells were plated in the upper chamber and 50,000 (1:1 ratio) or 200,000 (4:1) neutrophils were added. Where indicated, GM6001 (25 μmol/L) and MMP9 (1 μmol/L) inhibitors were added. D, UMSCC47 invasion in the presence of an EGF gradient (n = 6, ANOVA P < ; Tukey test *, P < 0.05; **, P < 0.01; ***, P < 0.001).
Judah E. Glogauer et al. Cancer Immunol Res 2015;3:
©2015 by American Association for Cancer Research