1. BAF57 binds AR DBD/hinge region.
BAF57 binds AR DBD/hinge region. A, immobilized GST or GST-BAF57 was incubated with [35S]Met-labeled AR mutants or CD44 (negative control) overnight at 4°C, washed, and subjected to SDS-PAGE. Five percent input and bound protein were detected by autoradiography. B, immobilized GST or GST-AR fusions were incubated with [35S]Met-labeled BAF57 and pull-down analysis was carried out as in A.
Kevin A. Link et al. Cancer Res 2008;68:
©2008 by American Association for Cancer Research