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SCX chromatography at low pH permits strong enrichment of phosphopeptides of distinct solution charge states.A, the number of phosphopeptides (blue triangles)

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Presentation on theme: "SCX chromatography at low pH permits strong enrichment of phosphopeptides of distinct solution charge states.A, the number of phosphopeptides (blue triangles)"— Presentation transcript:

1 SCX chromatography at low pH permits strong enrichment of phosphopeptides of distinct solution charge states.A, the number of phosphopeptides (blue triangles) and nonphosphorylated peptides (red squares) identified from gel region 1 (see Fig. 2, A and B) were plotted against their SCX elution time, and their ratio to each other is overlaid (green stars). SCX chromatography at low pH permits strong enrichment of phosphopeptides of distinct solution charge states.A, the number of phosphopeptides (blue triangles) and nonphosphorylated peptides (red squares) identified from gel region 1 (see Fig. 2, A and B) were plotted against their SCX elution time, and their ratio to each other is overlaid (green stars). Note distinct scales for each plot. B, phosphopeptides from the entire dataset show distinct solution charge state distributions in early SCX eluates. Bryan A. Ballif et al. Mol Cell Proteomics 2004;3: © 2004 The American Society for Biochemistry and Molecular Biology

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