1. Frequency of misrepair studies with increasing concentrations of antibodies against NHEJ repair proteins.
Frequency of misrepair studies with increasing concentrations of antibodies against NHEJ repair proteins. A, graph of the mean number of colonies derived from LacZα reactivation assays after incubation of nuclear extracts with increasing concentration of antibodies to NHEJ proteins. Antibodies are represented by the following symbols: HL60 + anti-Ku86 ( ), HL60 + anti-Ku86 + blocking peptide (black cross), HL60 + anti-Ku70 (▪), and HL60 + anti-DNA-PKcs (▴). B, misrepair frequencies after incubation of nuclear extracts with anti-Ku86: HL60 + anti-Ku86 ( ), HL60 + anti-Ku86 + blocking peptide (black cross), AML extract + anti-Ku86 ( , ····), and normal CD34+ extract + anti-Ku86 (⋄). C, misrepair frequencies after incubation of nuclear extracts with anti-Ku70: HL60 + anti-Ku70 (▪), HL60 + anti-Ku70 + blocking peptide (black cross), AML extract + anti-Ku70 (▪, ····), and CD34+ extract + anti-Ku70 (□). D, misrepair frequencies after incubation of nuclear extracts with anti-DNA-PKcs: HL60 + anti-DNA-PKcs (▴), HL60 + anti-DNA-PKcs + blocking peptide (black cross), AML extract + anti-DNA-PKcs (▴, ····), and CD34+ extract + anti-DNA-PKcs (▵).
Terry J. Gaymes et al. Cancer Res 2002;62:
©2002 by American Association for Cancer Research