1. Circulating leukocytes are undetectable in blood vessels of solid tumors in RIP1-Tag2 mice even after IL-15/IL-15Rα complex treatment.
Circulating leukocytes are undetectable in blood vessels of solid tumors in RIP1-Tag2 mice even after IL-15/IL-15Rα complex treatment. Histologic analysis of blood vessels in parenchymal tissues and tumors of IL-15/IL-15Rα complex treatment and control RIP1-Tag2 mice. A and B, left, organs were fixed and processed for H&E staining. Images show representative histology of liver, kidney, exocrine pancreas, and pancreatic tumors in control and IL-15/IL-15Rα complex–treated RIP1-Tag2 mice. Right, quantification of leukocytes in blood vessels of liver, kidney, exocrine pancreas, and pancreatic tumors in control and IL-15/IL-15Rα complex–treated RIP1-Tag2 mice from two independent experiments (n = 7 individual mice per condition). C, immunofluorescence microscopic analysis of CD8+ T cells in tumors of IL-15/IL-15Rα complex–treated and control RIP1-Tag2 mice. Tumors were excised 48 h after treatment with IL-15/IL-15Rα complexes and frozen in OCT. Cryosections from pancreata of treated and control mice were stained with DAPI (blue) and monoclonal antibodies to CD8 (red) and the pan-blood vessel marker, CD31 (green). Left, images (×20) are representative of more than four independent experiments. Right, areas in white boxes are magnified.
Mathieu Epardaud et al. Cancer Res 2008;68:
©2008 by American Association for Cancer Research