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NKT cell recognition of CD1d1 molecules is inhibited by treatment with ascites.
NKT cell recognition of CD1d1 molecules is inhibited by treatment with ascites. LCD1d1wt cells were incubated for 4 h in the presence or absence of the indicated concentrations of ascites from patient OC-47. The cells were then cocultured with the Vα14+ NKT cell hybridomas (A) DN32.D3 and (B) N38-2H4, and (C) Vα14− NKT cell hybridoma N37-1A12, and the Vα14+ NKT cell hybridoma (D) N38-2C12. NKT cell recognition of CD1d1 was assessed by measuring IL-2 production in the supernatants by ELISA. The results are representative of two experiments with patient OC-47 ascites. Similar results were obtained when the experiment was done with ascites from another patient (OC-40). Tonya J. Webb et al. Clin Cancer Res 2008;14: ©2008 by American Association for Cancer Research
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