1. Establishment of MajSAT RNA–expressing mice.
Establishment of MajSAT RNA–expressing mice. A, A schematic of the transgene construct. Approximately three tandem repeats of MajSAT RNA core sequences were inserted between the EF1α promotor and SV40 polyA signal sequences. B, Confirmation of MajSAT RNA expression by the TRAP-ddPCR method. MajSAT RNA levels were measured from 100 ng total RNA extracted from the pancreatic tissues and livers of wild-type and MajSAT RNA–expressing mice. The absolute copy number of MajSAT RNA represents the mean ± SE of four mice each. *, P < C, Pathologic images of MajSAT RNA–expressing mice. Representative H&E images are shown. The left six panels show the images of the pancreas, liver, and kidneys from wild-type mice, and the right panels show images from MajSAT RNA–expressing mice. All mice were 2 years old (n = 5 for wild and n = 8 for MajSAT). Scale bar, 100 μm.
Takahiro Kishikawa et al. Mol Cancer Res 2018;16:
©2018 by American Association for Cancer Research