1. Badger Connect Inaugural Research Services Fair Workshop
Thursday, May 3, 2018
Human Cell Line Authentication
William M. Rehrauer, PhD
Associate Professor, UWSMPH Department of Pathology
Co-Director of the Translational Research Initiatives in Pathology (TRIP) Lab

3. TRIP: Human Cell Line Authentication Service
Sample Types Accepted
DNA
Cells
Maximum Turnaround Time
10 working days
Cost
$125 DNA sample
$135 Cell sample
Interpretative Report
Identification
Authentication
Contamination
(2% Sensitivity)
Analyses relative to:
Publication(s)
Repository(s)
Baseline
Passage Monitoring
PowerPlex® 16 HS System
CSF1PO
D5S818
D21S11
PENTA_D
TH01
TPOX
D13S317
D7S820
D18S51
D16S539
D8S1179
D3S1358
FGA
VWA
AMEL
PENTA_E
Analysis of 15 Microsatellite (STR) Loci
and Amelogenin (Gender Specific Loci)

4. TRIP: Human Cell Line Authentication Service
PowerPlex® 16 HS System
CSF1PO
D5S818
D21S11
PENTA_D
TH01
TPOX
D13S317
D7S820
D18S51
D16S539
D8S1179
D3S1358
FGA
VWA
AMEL
PENTA_E
Analysis of 15 Microsatellite (STR) Loci
and Amelogenin (Gender Specific Loci)
Total ( ): 1,705
UW Submissions:
2.5% potentially contaminated
26.3% <55% match (misidentified)