1. Representative FFPE sections of subcutaneous xenografts derived from HBEC3p53,KRAS and HBEC3p53,KRAS,MYC.
Representative FFPE sections of subcutaneous xenografts derived from HBEC3p53,KRAS and HBEC3p53,KRAS,MYC. HBEC3p53,KRAS and HBEC3p53,KRAS,MYC formed subcutaneous tumor reflective of naturally arising lung carcinomas with adenosquamous differentiation (top), adenocarcinoma (middle left), and squamous differentiation (bottom left), as well as undifferentiated large cell carcinomas, some of which also exhibited a giant cell component (middle and bottom on right). Squamous and adenocarcinoma differentiation was confirmed with p63 and mucicarmine and/or Alcian Blue PAS staining, respectively. The example of adenosquamous cell carcinoma (top) clearly shows dual differentiation of peripheral squamous/basal-like cells (p63+/mucin−) and central glandular cells (p63−/mucin+). Muc, mucicarmine; AB-PAS, Alcian Blue PAS. Original magnification of images at ×10 except adenosquamous H&E and P63 (×20); large cell carcinoma with giant cell component H&E (×20); and large cell carcinoma H&E (×40).
Mitsuo Sato et al. Mol Cancer Res 2013;11:
©2013 by American Association for Cancer Research