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Supplementary Fig. 1.

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Presentation on theme: "Supplementary Fig. 1."— Presentation transcript:

1 Supplementary Fig. 1

2 Supplementary Fig. 2 H1048 Dox (2h) - + + + +
Time after Dox (h) Monoub p53 Higher exposure of Fig. 2a p53

3 * Supplementary Fig. 3 Monoub p53 p53 Actin a. WI38 b. WI38 - - + + +
Dox (2h) Caffeine Pcmv bam 273H MDM2 Hrs after dox pulse - + + + - - + + + - - + + + Dox (2h) Caffeine Pcmv bam 273H MDM2 Time after dox (h) - - - - - - + + + + + + - - - - - - - - - + + + + + + - - - - - + - - - - - + + + + + - - - - - - - - - - + + + + + - + + + + + - - - - - + + + + - + + + + - + + + + + - + + + + + - - - - - + + + + - + + + + Monoub Mutp53 Monoub p53 * Mutp53 p53 Actin Actin p-Ser15 p53 c. WI38 Hsp70 Dox (2h) 273H 273HSer15A MDM2 Time after dox (h) - + + + - + + + - + + + - - - - + + + + - - - - - - - - - - - - + + + + - - - - + + + + + + + + Monoub p53 p53 * Actin

4 Supplementary Fig. 4

5 Supplementary Fig. 5

6 Supplementary Fig. 6

7 Supplementary Fig. 7

8 Supplementary Fig. 8

9 Supplementary Fig. 9

10 Supplementary Fig. 10 ABC1 H1048 H1048 a. No color GFP 0h GFP 3h
shMdm2 0h shMdm2 3h p53 level 110 83 55 28 134 101 67 34 121 91 60 30 Count ABC1 b. No color No caffeine 7h caffeine H1048 p53 level 254 191 127 64 Count p53 level 213 159 106 53 Count No color 7h caffeine + MG132 H1048

11 a. Supplementary Fig. 11 d. H1975 U2OS b. MDA MB 231 c. WI38 Caffeine
No color p53 level No caffeine Caffeine H1975 Count 525 394 262 131 p53 level No color No caffeine Caffeine U2OS 111 83 55 28 Count b. MDA MB 231 p53 level 462 346 231 115 Count No color No caffeine Caffeine WI38 p53 level 230 173 115 58 Count c. No color No caffeine 7h caffeine

12 Supplementary Fig. 12 H1048 control siRNA ATM siRNA ATM Actin

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A b Supp. Figure 1: Anoikis-resistance of MDA-MB-231 is dependent on PI3Kγ (a) Cells were prevented from attaching for 48 hours in the presence of DMSO,

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ScrMMP-1 Supplementary Fig. 1, Ganguly et al. 0 25 50 75 100 ScrMMP-3 ScrMMP-3MT1-MMP 100 m Relative Invasion 0 25 50 75 100 Scr MMP-1 100 m abc Scr Scr.

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Fig. 4-1, p Fig. 4-2, p. 109 Fig. 4-3, p. 110.
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-0.8 -0.6 -0.4 -0.2 0 0.2 0.4 05101520253035 -0.8 -0.6 -0.4 -0.2 0 0.2 0.4 0123456789 Supplementary Fig. 1 APACHE II score ( transformed) SOFA score (transformed)

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