1. BHLHB2 is part of a protein–DNA complex associated with the proximal E-box element of Bdnf promoter 4.
BHLHB2 is part of a protein–DNA complex associated with the proximal E-box element of Bdnf promoter 4. Autoradiograph showing a specific band indicative of a DNA–protein binding complex in NIH3T3 cell nuclear extracts to a 33P-labeled double-stranded DNA probe based on the 22 bp rat Bdnf promoter 4 IS region (−110 to −87). Addition of unlabeled competitor DNA caused the binding activity to disappear in a concentration-dependent manner. Other consensus E-box containing competitor DNAs were also effective in eliminating the specific band. When a polyclonal BHLHB2 peptide antibody was incubated with the DNA–protein complex, the migration of the specific band was further retarded (shown by the arrow labeled “supershift”). No supershift was observed when the DNA–protein complex was incubated with a CREB-specific antibody.
Xueying Jiang et al. J. Neurosci. 2008;28:
©2008 by Society for Neuroscience