1. Crebbp deficiency accelerates B-cell lymphoma development in mice.
Crebbp deficiency accelerates B-cell lymphoma development in mice. A, Kaplan–Meier curve of C57BL/6 mice transplanted with VavP-Bcl2 HPCs transduced with MSCV-GFP retroviral vector alone (GFP, black, n = 31), or containing shRNAs against Crebbp (red, n = 34). Statistical significance of survival difference was determined by the log-rank test between shCrebbp and vector alone. B, Representative flow cytometry histograms showing the GFP-positive cell percentage of the preinjection HPCs and the splenic murine lymphoma cells that derived from the same HPCs. C, Dot plot representing the GFP-positive cell percentage in the splenic murine lymphoma cells in individual recipient animal. The mean and SEM were represented for each transplant group. Statistical significance was determined by Mann–Whitney test. D, Hematoxylin and eosin (H&E), B220, CD3, and Ki67 staining of spleen tissues extracted from recipient mice upon sacrifice. E, H&E and B220 staining of kidney and lung tissues extracted from recipient mice upon sacrifice.
Yanwen Jiang et al. Cancer Discov 2017;7:38-53
©2017 by American Association for Cancer Research