1. Synteny analysis of the S. meliloti genome.
Synteny analysis of the S. meliloti genome. The S. meliloti Rm1021 genome was compared with the genomes of the more distantly related organism S. fredii NGR234 (A) and the closely related organism S. medicae WSM419 (B). Putative orthologous genes between species were identified by performing BLAST bidirectional best-hit analyses using the proteomes. BLAST bidirectional best hits with an E value of ≤1 × 10−100 and ≥50% identity were linked to the corresponding gene, and their position was mapped on the genome. Each putative ortholog between genomes is connected by a line and color coded based on replicon type (black, chromosome to chromosome; yellow, chromid to chromid; purple, megaplasmid to megaplasmid; blue, chromosome to chromid; red, chromosome to megaplasmid; green, chromid to megaplasmid; orange, plasmid to anywhere. * indicates a 69-kb region of the S. fredii chromosome that translocated to an ancestor of the pSymB chromid through a single translocation event, resulting in the transfer of two essential genes to pSymB (41). Methods are provided in the supplemental material.
George C. diCenzo, and Turlough M. Finan Microbiol. Mol. Biol. Rev. 2017; doi: /MMBR