1. Reversible and Irreversible Drought-Induced Changes in the Anther Proteome of Rice (Oryza sativa L.) Genotypes IR64 and Moroberekan 
Liu Jian-Xiang , Bennett John  
Molecular Plant 
Volume 4, Issue 1, Pages (January 2011)
DOI: /mp/ssq039
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Relative Water Content (RWC) in Flag Leaf of IR64 and Moroberekan.
Plants were not watered from 3 d before heading for 5 d and re-watered until heading occurred. After re-watering, heading was delayed in both varieties comparing to well watered condition. Arrows indicate the heading dates. RWC decreased to comparable extents in both genotypes and largely reversed after 2–3 d re-watering. Bars indicate the standard error (n = 3).
Molecular Plant 2011 4, 59-69DOI: ( /mp/ssq039)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

3. Figure 2 Starch and ABA Content in Anthers of IR64 and Moroberekan.
Water was withheld from 3 d before heading in well watered plants (WW-3DBH) for 5 d, and restored thereafter.
(A) High ABA content was observed at the end of water cessation (DS) and at heading after re-watering (RW-H) in both genotypes.
(B) Starch accumulated from 3 DBH to heading in well watered plants (WW-H). Starch accumulation was inhibited only in IR64 by drought and starch degradation was observed in both genotypes during re-watering. Bars indicate the standard error (n = 3).
Molecular Plant 2011 4, 59-69DOI: ( /mp/ssq039)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

4. Figure 3
Representative 2-D Gel Maps of Rice Anthers/Pollen and an Illustration for Comparisons.
Total proteins were extracted from anthers at heading or pollen grains at anthesis in c.v. IR64 plants that were drought-stressed and re-watered.
(A) Ninety-three differentially displayed proteins are marked with spot number. Six protein spots that were only detected in c.v. Moroberekan anthers are marked with open circles according to their migration patterns on Moroberekan gels.
(B) Among the 87 protein spots in c.v. IR64 anther proteome shown in Figure 3A, only 12 spots that were not detected in the pollen of drought-stressed c.v. IR64 are marked with open circles according to their migration patterns on anther gels.
(C) Anther samples were collected at 3 d before heading (WW-3DBH), at heading (WW-H) under well-watered conditions, at the end of stress (DS), and at heading after re-watering (RW-H); stars indicate the sampling time points. Protein expression levels were first compared between WW-H and RW-H to determine the recovery capability after drought and re-watering; then, protein expression levels were compared between WW-H and WW-3DBH to observe the developmental regulations under non-stress conditions.
Molecular Plant 2011 4, 59-69DOI: ( /mp/ssq039)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

5. Figure 4
Examples of Differentially Displayed Protein Spots by Drought and/or Re-Watering in the Anther Proteome of c.v. IR64 and c.v. Moroberekan.
(A) Gel images showing the expressions of six protein spots in two genotypes before or after drought treatment. All enlargements are from silver-stained gels. Differentially displayed proteins are indicated with arrows.
(B) Relative protein abundance (volume) of each spot was quantified by software Melanie III. Bars indicate the standard error (n = 4). WW-3DBH, 3 d before heading under well watered conditions2; WW-H, heading under well watered conditions; DS, end of water cessation for 5 d; RW-H, heading after drought and re-watering.
Molecular Plant 2011 4, 59-69DOI: ( /mp/ssq039)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

6. Figure 5 Glycosylation Detection of Os08g12160.
Spots 40, 41, 42, and 55 are highly glycosylated as revealed by Periodic acid–Schiff's (PAS) staining. Anthers were collected from drought-stressed c.v. IR64 plants and total proteins were resolved by 2-D electrophoresis.
Molecular Plant 2011 4, 59-69DOI: ( /mp/ssq039)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions