1. Fig. 5 Microfluidic platform for cell phenotype and gene function analysis.
Microfluidic platform for cell phenotype and gene function analysis. (A) MCF7 cells delivered with plasmids encoding only Cas9 protein or both sgPten and Cas9 protein were cultured for 7 days and then analyzed by Western blotting with the indicated antibodies. Actin was used as a loading control. The symbol * indicates long exposure. (B) Cells (5 × 104) from (A) were seeded in 60-mm dishes in complete medium and cultured for 7 days. Cells were trypsinized and collected for cell count in a Countess II FL Automated Cell Counter (Life Technologies) daily for 7 days. Error bars indicate SEM (n = 3). *P < determined by Student’s t test. (C) HeLa cells delivered with plasmids encoding only Cas9 protein or both sg53BP1 and Cas9 protein were cultured 7 days. Then, the cells were treated with 1 μM CPT for 2 hours and then examined by immunostaining with anti-53BP1 antibodies (red). Scale bar, 10 μm. DAPI, 4′,6-diamidino-2-phenylindole. (D) Survival rate of HeLa cells from (C) after control or CPT treatment was assessed by colony survival assay. Error bars indicate SEM (n = 3).
Xin Han et al. Sci Adv 2015;1:e
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