1. Small-molecule inhibitor reduced porphyrin production in P
Small-molecule inhibitor reduced porphyrin production in P. acnes, and its inhibition was counteracted by vitamin B12 supplementation in acne-associated type IA-2 strains.
Small-molecule inhibitor reduced porphyrin production in P. acnes, and its inhibition was counteracted by vitamin B12 supplementation in acne-associated type IA-2 strains. P. acnes strains were cultured in medium with inhibitor LA (0.1 mg/ml) (grey bars) or without LA (white bars) or with both LA and vitamin B12 (10 µg/ml) added (black bars). LA significantly reduced porphyrin biosynthesis in all strains except for HL001PA1 (P < ). Vitamin B12 supplementation counteracted the inhibition of porphyrin biosynthesis by LA in acne-associated type IA-2 strains, but not in health-associated type II strains. Each bar represents the porphyrins produced by each strain normalized to the bacterial culture density. The means plus standard errors (error bars) of data obtained from at least three independent experiments with at least three replicates each are shown.
Tremylla Johnson et al. mSphere 2016; doi: /mSphere