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Role of Art v 3 in pollinosis of patients allergic to Pru p 3

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Presentation on theme: "Role of Art v 3 in pollinosis of patients allergic to Pru p 3"— Presentation transcript:

1 Role of Art v 3 in pollinosis of patients allergic to Pru p 3
Jaime Sánchez-López, MD, Leticia Tordesillas, PhD, Mariona Pascal, PhD, Rosa Muñoz-Cano, PhD, María Garrido, BSc, Maria Rueda, MD, Ramón Vilella, PhD, Antonio Valero, PhD, Araceli Díaz-Perales, PhD, César Picado, PhD, Joan Bartra, PhD  Journal of Allergy and Clinical Immunology  Volume 133, Issue 4, Pages e3 (April 2014) DOI: /j.jaci Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 NAPTs with Art v 3. Changes in AR (A), total VAS scores (B), and nasal cysLT levels (C) in patients (red dots) and control subjects (white dots) after NAPTs with Art v 3. Data are expressed as medians and ranges. Significant differences between groups are indicated as follows: *P < .001, **P < .01, and ***P < .05. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 IgE immunoblotting of mugwort extract with the sera of the 15 patients with Pru p 3 allergy. Patient numbers correspond to those shown in Tables I and II. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 NAPTs with mugwort pollen extract. Changes in AR (A) and total VAS scores (B) after NAPTs with mugwort. Data are expressed as medians and ranges. Significant differences between postdiluent values and values 15 and 30 minutes after challenge are indicated as follows: ***P < .05. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 NAPTs with Pru p 3. Changes in AR (A), total VAS scores (B), nasal tryptase levels (C), and nasal cysLT levels (D) of patients (red dots) and control subjects (white dots) after NAPTs with Pru p 3. Data are expressed as medians and ranges. Significant differences between groups are indicated as follows: *P < .001, **P < .01, and ***P < .05. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 ELISA inhibition with Art v 3 (A) and Pru p 3 (B) in the solid phase. A serum pool was preincubated with the corresponding inhibitor (Art v 3, white dots; Pru p 3, red dots) at different concentrations (0.001, 0.01, 0.1, 1, 5, 10, and 15 μg/mL). Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E1 Schematic representation of sIgE detection by using a microarray. Columns represent each subject, and rows represent each allergenic component included in the array. Levels are expressed as ISU units. Color code: gray, less than the cutoff of 0.3 ISU units; yellow, 0.3 to 0.9 ISU units; orange, 1 to 14.9 ISU units; and red, greater than 15 ISU units. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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