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Adhesion of human endometrial fragments to peritoneum in vitro
Patrick G Groothuis, Ph.D., Carolien A.M Koks, M.D., Anton F.P.M de Goeij, Ph.D., Gerard A.J Dunselman, M.D., Jan Willem Arends, M.D., Johannes L.H Evers, M.D. Fertility and Sterility Volume 71, Issue 6, Pages (June 1999) DOI: /S (99)
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FIGURE 1 Scanning micrographs showing intact (A) and damaged (B) mesothelium. Bars = 10 μm. Damage of the mesothelium results in exposure of the basement membrane. Fertility and Sterility , DOI: ( /S (99) )
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FIGURE 2 Adherence of endometrial fragments to peritoneum. (A), Scanning micrograph showing an endometrial fragment adherent to a damaged area of the peritoneum (Bar = 10 μm). (B), Light micrograph of 1-μm cross-sections of epon-embedded peritoneum of the mesothelium and underlying layers with adhering endometrial fragments, stained with toluine blue. Transmission micrographs of this area show that adhesion to the peritoneum occurs only at locations where the mesothelial lining was impaired (C) (Bar = 5 μm). EF = endometrial fragment; M = mesothelial cells/mesothelium; ECM = extracellular matrix. Fertility and Sterility , DOI: ( /S (99) )
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FIGURE 2 Adherence of endometrial fragments to peritoneum. (A), Scanning micrograph showing an endometrial fragment adherent to a damaged area of the peritoneum (Bar = 10 μm). (B), Light micrograph of 1-μm cross-sections of epon-embedded peritoneum of the mesothelium and underlying layers with adhering endometrial fragments, stained with toluine blue. Transmission micrographs of this area show that adhesion to the peritoneum occurs only at locations where the mesothelial lining was impaired (C) (Bar = 5 μm). EF = endometrial fragment; M = mesothelial cells/mesothelium; ECM = extracellular matrix. Fertility and Sterility , DOI: ( /S (99) )
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FIGURE 2 Adherence of endometrial fragments to peritoneum. (A), Scanning micrograph showing an endometrial fragment adherent to a damaged area of the peritoneum (Bar = 10 μm). (B), Light micrograph of 1-μm cross-sections of epon-embedded peritoneum of the mesothelium and underlying layers with adhering endometrial fragments, stained with toluine blue. Transmission micrographs of this area show that adhesion to the peritoneum occurs only at locations where the mesothelial lining was impaired (C) (Bar = 5 μm). EF = endometrial fragment; M = mesothelial cells/mesothelium; ECM = extracellular matrix. Fertility and Sterility , DOI: ( /S (99) )
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FIGURE 3 Scanning micrograph showing monolayer formation of endometrial cells over a denuded peritoneal surface. Bar = 10 μm. EC = endometrial cells; bm = basement membrane. Fertility and Sterility , DOI: ( /S (99) )
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FIGURE 4 Scanning micrograph showing mesothelial cells partially overgrowing endometrial cells. Bar = 10 μm. M = mesothelial cells. S = stromal fibroblasts. Fertility and Sterility , DOI: ( /S (99) )
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