1. FGFR1 is dominantly expressed in mesenchymal-like KRAS-mutant lung cancer cell lines.
FGFR1 is dominantly expressed in mesenchymal-like KRAS-mutant lung cancer cell lines. A, expression of ERBB3, E-cadherin, and vimentin protein were analyzed by Western blotting of lysates from KRAS-mutant lung cancer cell lines. Actin is a loading control. Independent experiments were performed three times, and a representative result is shown. B and C, NCI-H358 cells were treated with TGFβ1 (4 ng/mL) or PBS for 14 days in order to induce EMT. B, RNA was extracted from each cell and gene expression profiles were compared. The list of 30 genes most upregulated following TGFβ1 treatment is shown. C, lysates were extracted from each cell and immunoblotted with antibodies against indicated RTKs and EMT markers. Actin was used as a loading control. Independent experiments were performed three times, and a representative result is shown. D, unsupervised hierarchical clustering of 39 KRAS-mutant NSCLC cell lines from the CCLE database. A total of 2,635 genes were analyzed which show more than 5-fold change among cell lines with a median expression value of 6 or more. E and F, scattered plot analysis showing the relationship between ERBB3 and CDH1 (E) and an inverse relationship between FGFR1 and CDH1 (F). P < 0.001, both by linear regression analysis.
Hidenori Kitai et al. Cancer Discov 2016;6:
©2016 by American Association for Cancer Research