1. Identification of compounds that enhance TMZ cytotoxicity in melanoma cells by screening the Spectrum Collection library.
Identification of compounds that enhance TMZ cytotoxicity in melanoma cells by screening the Spectrum Collection library. A. Screening of the Spectrum Collection library to identify novel agents that enhance TMZ efficacy in cultured melanoma cells. Twenty-four hours after seeding into 96-well plates, cell cultures in 1 plate was treated with each library compound alone at 1 μmol/L, whereas cells in the parallel plate were treated with a combination of 1 μmol/L library compound and 50 μg/mL TMZ. Screening was done with SK-MEL-19 cells in 96-well plates by MTT assay after a 72 h treatment. Positive candidates were later reconfirmed in triplicate. B. Chemical structures of six hit compounds confirmed enhanced TMZ activity and induced greater inhibition of cell growth.
Ming Chen et al. Mol Cancer Res 2009;7:
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