1. Increased spike ADP in pilocarpine-treated rats is sensitive to spermine.
Increased spike ADP in pilocarpine-treated rats is sensitive to spermine. A, Current-clamp recordings from CA1 neurons in hippocampal slices under the indicated conditions. Threshold straddling brief (5 ms) depolarizing current injections (depicted below the voltage traces) were applied via the somatic recording patch pipette. Just subthreshold responses (gray traces) were subtracted from just suprathreshold responses, and the ADP area calculated. Insets, Truncated action potentials for enhanced ADP presentation. B, Summary plot of spike ADP area under the indicated condition. The spike ADP was significantly increased in pilocarpine-treated animals, compared with sham-control animals, and this increase was sensitive to intracellular spermine (F(3,36) = 5.27, p = ). *p < 0.05 (one-way ANOVA followed by Newman-Keul's multiple-comparison test). C, Current-clamp recordings from CA1 neurons in hippocampal slices under the indicated conditions. Long (200 ms) depolarizing current injections (depicted below the voltage traces) were applied via the somatic recording patch pipette. Note the irregular firing pattern of the neuron from a pilocarpine-treated rat, showing doublet action potentials or burst firing at higher depolarization. This bursting behavior was significantly different from the regular firing in the other groups of animals (χ2 test, p = ).
Michel Royeck et al. J. Neurosci. 2015;35:
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