1. Structure and Function
The Cell Membrane
Structure and Function

2. Agenda: 5/6 Grab a paper from the basket! Order of Operations:
Egg Lab / Types of Solutions mini poster
Finish test (in computer lab)
Cell Transport Notes (from website)
Time for vocab project
Reminders: Mock EOC on Thursday, 3rd section of vocab project due 5/24!

3. Cell Membrane Functions
Cell membrane surrounds the cell, regulating the transport of materials into and out of the cell

4. Cell Membrane Structure
Lipid Bilayer: a double layer of phospholipids that make up the cell membrane

5. Phospholipid Phosphate Head: Two Fatty Acid Tails: Two Layers Polar
Hydrophilic (attracted to water)
Two Fatty Acid Tails:
Nonpolar
Hydrophobic (avoids water)
Two Layers
Outsides: Polar Heads
Inside: Nonpolar Tails

6. Other lipids ? Lipids Fatty acids Fats and waxes Steroids Cholesterol
Testosterone

7. Cell Membrane Structure
Phospholipids are arranged so hydrophilic (water loving) ends face outside and hydrophobic (water fearing) tails create the interior

8. Cell Membrane Structure
Phospholipid Bilayer
Transmembrane Proteins
Cholesterol (a steroid)
Glycoprotein and Carbohydrate

9. Cell Membrane Structure
Transmembrane Proteins
Job:
Attachment point between cells
Anchor cell in place
Create channels for large and/or polar molecules to pass

10. Cell Membrane Structure
Cholesterol
Job:
Maintains fluidity of cell membrane

11. Cell Membrane Structure
Glycolipids and Carbohydrates
Job:
Serve as cell markers for identification

12. Cell Membrane Structure
The Fluid Mosaic Model
The membrane is dynamic – molecules are constantly moving

13. Selectively Permeable
The cell membrane is selectively permeable, meaning some substances can pass through the cell membrane, but others can’t!
“Permeable” is the ability for something to pass through

14. You are working on one or the other!
EGG LAB INSTRUCTIONS
You are working on one or the other!
Strain egg over sink
Rinse egg and cup
Make observations about egg on lab paper
Place egg back in cup and pour syrup over the egg
Replace plastic wrap and place cup on back counter
Wipe tables down with Clorox wipes and WASH YOUR HANDS!
TYPES OF SOLUTIONS
For each type of solution, WRITE THE TERM, DEFINE THE TERM, and then draw the ANIMAL CELL and the PLANT CELL on page 186 in the GREEN BIO textbook.
Make sure to label the direction(s) that water is moving!

15. Passive Transport Osmosis and Diffusion
Raisin demonstration needs to be set up first to allow time for osmosis to take place.

16. Selectively Permeable
The cell may transport substances
Passive Transport: No Energy required
Active Transport: Uses energy (ATP)

17. Passive Transport
Movement of a substance into/out of a cell without using energy
HIGH  LOW concentration
3 Types
Simple Diffusion
Facilitated Diffusion
Osmosis

18. About Concentration…
We just learned that substances move from HIGHLOW concentration in passive transport…but what do we mean by concentration?
Means amount dissolved in a container
How do you like your sweet tea?
a. Unsweet (low concentration of sugar)
b. Sweet
c. Super sweet(high concentration of sugar)
Solute = Substance dissolved in water
The sugar is dissolved at different concentrations. Unsweet tea has a low concentration of sugar, and super sweet tea has a high concentration of sugar.

19. Concentration Gradients
A concentration gradient is the difference in the concentration, or amount, of something in a space
In the box below, the dots have a higher concentration on the left than the right, so they begin movement to the right
When the concentration gradient is the same throughout the area, it is in a dynamic equilibrium
Particles are moving from one side to the other, but at the same rate.
After this slide, lead into the food coloring discussion. Have them write down observations after they add food coloring to water. Then do diffusion slide and ask them to observe the water again. They should be able to reach the conclusion that the food coloring is diffusing to create equilibrium. The students can stir the water to help equilibrium be reached faster.
Picture:

20. Passive Transport: Diffusion
Diffusion is when a substance moves from an area of high concentration to low concentration area
Diffusion will continue until balanced/equal “at equilibrium”

21. Facilitated Diffusion
Diffusion of large molecules through special protein channels
Ex. Ions, glucose
Still happens without energy being used!

22. Passive Transport: Osmosis
Osmosis is the diffusion of water across a selectively permeable membrane
Water moves from an area of high concentration (lots of water) to low concentration (little water)
Passive Transport:
Water moves with its concentration gradient
No energy is required
Picture: scienceaid.co.uk

23. About Osmosis
Water can move into or out of the cell, depending on the environment (solution) that the cell is in
Solvent + Solute = Solution
Solvent does the dissolving (ex. Water)
Solute substance dissolved (ex. Salt)

24. EQUAL amounts of solute in/out of cell.
Describing Solutions
Isotonic Solution
“same or equal”
Hypertonic Solution
“above/over/higher”
Hypotonic
Solution
“below/under/lower”
EQUAL amounts of solute in/out of cell.
HIGHER solute outside.
LOWER solute outside.

25. How Osmosis Works Isotonic Hypertonic Hypotonic “same or equal”
“above/over/higher”
Hypotonic
“below/under/lower”
Water in/out.
Plant Cells
Flaccid, Central Vac. not full
Animal Cell**
No Change to cell
Water out
Plasmolysis, Cell death
Animal Cell
Dehydration, Cell shrivels
Water in.
Plant Cells**
Turgid, Central Vac. Full
Lysed, Cell bursts

26. Osmosis Onion in Salt Water Blood Cells Same Swelling Shrinking
If time permitting, discuss the pH of blood at 7.3 norm. The blood cells have a buffer that tries to regulate the cell at 7.3. If something happens that makes it too acidic or basic, the buffers can fail and cause problems. In the case of the fish, the pH became to high in the water for them to handle. Diffusion caused water to swell and rupture their cells, thus causing them to die. Fish generally have higher solute concentration inside their bodies, and the drastic change in pH alters this and affects them negatively.
Picture:

27. Check for understanding
After an ice storm, road crews salt the roadways to melt ice and increase traction for cars.
What happens to the plants on the side of the road? Explain why.

28. Isotonic, Hypotonic, or Hypertonic?
10% NaCL Environment
90 % H2O
CELL
10% NaCL
90 % H2O

29. Water Goes?
Isotonic-Water goes in/out (balanced); cell stays the same!
10% NaCL Environment
90 % H2O
CELL
10% NaCL
90 % H2O

30. Isotonic, Hypotonic, or Hypertonic?
10% NaCL Environment
90 % H2O
CELL
20% NaCL
80 % H2O

31. Water Goes? Hypotonic-water goes IN! Cell swells! 10% NaCL Environment
90 % H2O
CELL
20% NaCL
80 % H2O

32. Isotonic, Hypotonic, or Hypertonic?
10% NaCL Environment
CELL
5% NaCL

33. Water Goes? Hypertonic-water goes OUT-Cell Shrinks!!
10% NaCL Environment
Often, you will just be given the solute concentration in the environment. What is the water concentration? Would it move in or out?
CELL
5% NaCL

34. Movement Requiring Energy
Active Transport
Movement Requiring Energy

35. Active Transport
Molecules move across the membrane AGAINST their concentration gradient
Moving LOW to HIGH
ENERGY is required! (imagine trying to push your way through a crowd vs. going with the flow…)
Two Types
Protein Pumps
Bulk Transport

36. Protein Pumps
Transport small molecules/ions AGAINST their concentration gradients
(LOW HIGH)
Uses ATP (cell energy)
Notice the ATP

37. Bulk Transport
Larger molecules (proteins, starch) are transported by vesicles that merge with the cell membrane
Exocytosis-contents leaving the cell through the membrane
Endocytosis-contents entering in vesicles (pinocytosis and phagocytosis)

39. Finish up egg lab! Strain egg liquid over sink
Throw away cup in big trash can
Place egg in a bowl and take it back to your seat!
Finish lab observations /definitions / conclusion / questions and turn in!
When finished with egg, throw it away in big trash can!

40. Egg Lab Procedure Pour liquid through strainer over sink
Pour as little of the syrup into the sink as possible!
Throw away cup with syrup (into big trash can)
Label a new cup with names of group members
Make observations about egg and place in new cup
Pour distilled water gently over the egg and place plastic wrap back over cup
While you are waiting for a sink:
Work on Cells Review Notes and Labeling
Work on Types of Solutions assignment from yesterday

41. Homeostasis Lab Groups 2-3 (NO MORE THAN 3!)
One person will volunteer to do jumping jacks and have vitals measured a certain points in the experiment.
Heart rate
Breathing rate
Perspiration level
Record data, answer lab questions with partner(s)
Graph if we have time???

42. Homeostasis
Maintaining Balance

43. Homeostasis
The process by which an organism’s internal environment is kept in equilibrium (stable) in spite of changes in the external environment
Examples:
Internal Temperature
Sweating removes excess body heat
Shivering increases heat production
Water balance
Kidneys adjust water amount in urine
A physical response to stress
Breathing/heart rate increases
Pupils dilate
Sweating)

44. Homeostasis Homeostasis requires energy
For active transport to deliver needed molecules
T0 rid cell of toxins
To move to avoid danger or find food

45. Homeostasis in Cells
Cells can maintain homeostasis in a number of ways…
Controlling substances that cross the membrane
Active and Passive transport)

46. Homeostasis in Cells
Cells can maintain homeostasis in a number of ways…
Buffers
Cells produce buffers to help maintain a constant pH.
Examples:
Human blood cells must maintain a pH of
Cells in stomach maintain a pH of 3
Cells in Small Intestine maintain a pH of 6
What is a buffer?
A chemical that can raise or lower the pH (it can behave like an acid or a base)

47. Yolk

48. Egg in Syrup
Layer of water over the syrup

49. Poured egg into strainer
Water / syrup into trash
Notice wrinkled appearance of egg

50. Notice decreased size of egg
Syrup

51. Egg in water

52. Homeostasis
Maintenance of homeostasis is vital for life!

53. Osmosis and Diffusion

54. Cells and Cell Transport Test Review!

55. Which cellular process is most closely related the presence of chloroplasts in eukaryotes?
Metabolism
Photosynthesis
Aerobic respiration
Lactic acid fermentation

56. Which of the following serves as the cell’s boundary from its environment?
Mitochondria
Cell membrane
Chloroplast
Channel protein

57. Where would you need more…
Mitochondria?
Chloroplasts?
Cell organelles can be more concentrated based on needs!

58. Which of the following is a function of the cell membrane?
Breaks down lipids, carbohydrates, and proteins in the body
Stores water, salt, proteins, and carbohydrates
Keeps the cell wall in place
Regulates which materials enter and leave the cell

59. The cell membrane contains channels and pumps that help move materials from one side to the other. What are these channels and pumps made of?
Carbohydrates
Lipids
Bilipids
Proteins

60. Diffusion is the movement of molecules from:
An area of low concentration to an area of high concentration
An area of high concentration to an area of low concentration
An area of equilibrium to an area of high concentration
All of the above

61. Diffusion occurs because:
Molecules constantly move and collide with each other
The concentration of a solution is never the same throughout a solution
The concentration of a solution is always the same throughout a solution
Molecules never move or collide with each other

62. When the concentration of molecules on both sides of the membrane is the same, the molecules will:
Move across the membrane to the outside of the cell
Stop moving across the membrane
Move across the membrane in both directions
Move across the membrane to the inside of the cell

63. The diffusion of water across a selectively permeable membrane is called:
Osmotic pressure
Osmosis
Facilitated diffusion
Active transport

64. An animal cell that is surrounded by fresh water will burst because the osmotic pressure causes:
Water to move into the cell
Water to move out of the cell
Solutes to move into the cell
Solutes to move out of the cell

65. Which means of particle transport requires input of energy from the cell?
Diffusion
Osmosis
Facilitated Diffusion
Active Transport

66. BioFacts /26
2. Energy released by the cells mitochondrion is stored as A. ATP B. glucose C. DNA
3. A cell’s volume grows faster than its surface area, so if a cell gets too large…
A. its surface area to volume ratio will decrease
B. its outer surface will harden like an eggshell
C. it will not be able to take in nutrients or expel waste
4. List the levels of organization from the simplest to the most complex.
5. List FOUR elements that ALL cells have in common.

67. BioFacts 3.3 2/28 6. Explain selectively permeable.
7. Explain Fluid Mosaic Model.
8. ___ ___ create channels for large and/or nonpolar substances to pass through the membrane.
9. Draw a label a phospholipid.
10. Define Homeostasis. (with root word knowledge)

68. BioFacts 3.4 3/1 11. Diffusion = 12. Osmosis =
13. List the 3 solutions related to osmosis and describe each (make the cell shrink, swell, or stay the same.)
14. Passive transport (such as diffusion and osmosis) is the movement of molecules from:
An area of high concentration to an area of low concentration
An area of low concentration to an area of high concentration
An area of equilibrium to an area of high concentration
All of the above

69. BioFacts /2
15. Identify the solution as iso, hyper, or hypotonic and draw arrows labeling the direction of water movement.
16. A cell is placed into a salt solution. The salt concentration is greater in the solution than inside the cell. Which describes what will most likely happen?
Water will flow into the cell by active transport
Water will flow into the cell by passive transport
Water will flow out of the cell by active transport
Water will flow out of the cell by passive transport
30% solute
70% H2O
80% H2O
20 % solute
40% solute
85% H2O
15% solute
70% H2O

70. BioFacts /5
17. If energy is needed to move materials into or out of a cell, what is most likely occurring?
Active transport
Passive transport
Osmosis
Diffusion
18. During strenuous exercise, the body temperature increases. The body responds to the increase in temperature by sweating, which helps to reduce the body temperature. Which is demonstrated in this situation?
Excretion
Metabolism
Homeostasis
Synthesis