1. Volume 3, Issue 3, Pages 563-575 (May 2010)
The Arabidopsis DSO/ABCG11 Transporter Affects Cutin Metabolism in Reproductive Organs and Suberin in Roots 
Panikashvili David , Shi Jian Xin , Bocobza Samuel , Franke Rochus Benni , Schreiber Lukas , Aharoni Asaph  
Molecular Plant 
Volume 3, Issue 3, Pages (May 2010)
DOI: /mp/ssp103
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
DSO/ABCG11 Expression and Polar Protein Localization in Reproductive Organs.
(A) Expression of pDSO/ABCG11:GFP in flower buds.
(B) Expression of pDSO/ABCG11:GFP in open flower. Note the strong expression detected in the petal epidermis and the vasculature.
(C) Expression of pDSO/ABCG11:GFP in developing seeds.
(D) Expression of GFP:DSO/ABCG11 under the control of its native promoter in the walking-stick stage embryo (epidermal expression is marked by arrows).
(E) Close-up view on GFP:DSO/ABCG11 expression under the control of its native promoter in the embryo epidermis. Note the distal mode of GFP expression (arrows).
(F) Expression of GFP:DSO/ABCG11 under the control of the native promoter in the endosperm (en) tissue of the developing seed.
In (D–F), the images were acquired through a GFP filter (I), chlorophyll filter (II), transmission (III) and (IV) was a merge between the GFP and chlorophyll filters.
Molecular Plant 2010 3, DOI: ( /mp/ssp103)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

3. Figure 2 Developmental Phenotypes of the dso-4 Mutant Lines.
(A) Loss of apical dominance and stunted growth in flowering dso-4 as compared to a wild-type (WT) plant.
(B) A defective flower of dso-4 compared to a WT flower. The hook-shaped silique in dso-4 is indicated by an arrow.
(C) Semi-sterility in dso-4 as compared to a WT plant inflorescence.
(D) Hook-shaped siliques are formed in dso-4 as compared to WT siliques.
(E) SEM picture illustrating the surface of a hook-shaped silique in dso-4. The region of silique bending is indicated by arrows.
(F) Fused seeds (indicated by an arrow) in the developing silique of dso-4.
(G) Typical seeds formed in a developing silique of WT plant.
Molecular Plant 2010 3, DOI: ( /mp/ssp103)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

4. Figure 3
Transcriptome Changes in the Stems and Leaves of the dso-4 Plants.
(A) A Venn diagram representing the number of transcripts that were down-regulated in either dso-4 plant leaves and stems or in both tissues.
(B) A Venn diagram representing the number of transcripts that were up-regulated in either dso-4 plant leaves and stems or in both tissues.
(C) Semi-quantitative RT–PCR expression analysis of selected transcripts in dso-4 and wild-type (WT) stems.
(D) Semi-quantitative RT–PCR expressing analysis of selected transcripts in dso-4 and WT leaves.
The UBIQ–UBIQUITINE gene served as a control for equal cDNA loading.
Molecular Plant 2010 3, DOI: ( /mp/ssp103)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

5. Figure 4
Altered Profile of Cutin Monomers in Flowers and Siliques of the dso-4 Lines.
(A) The cutin polyester profile of dso-4 and wild-type (WT) flowers.
(B) The cutin profile of dso-4 and WT siliques. Differential monomers are indicated by asterisks. The values indicate mean of three biological replicates ± stdev. * P < 0.05.
Molecular Plant 2010 3, DOI: ( /mp/ssp103)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

6. Figure 5
The Profile of Root Suberin Monomers in the dso-4 and Wild-Type Plants.
Differential monomers are indicated by asterisks. The values indicate a mean of three biological replicates ± stdev. * P < 0.05.
Molecular Plant 2010 3, DOI: ( /mp/ssp103)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

7. Figure 6
Alteration to Cuticle and Suberin-Related Gene Expression in the Stem and Root Tissues of the dso-4 Lines.
(A–E) Expression in stems.
(F, G) Expression in roots.
RQ, relative transcript level; the values indicate mean of three biological replicates ± SEM obtained by Quantitative RT–PCR analysis. * P < 0.05. See Table 1 for the annotation of encoded proteins.
Molecular Plant 2010 3, DOI: ( /mp/ssp103)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions