1. Pomalidomide decreases fibrosis in the lesion areas of the pancreas of KC mice.
Pomalidomide decreases fibrosis in the lesion areas of the pancreas of KC mice. A–D, Control mice or KC mice at an age of 8 weeks were treated with pomalidomide every day for 4 weeks (treatment scheme depicted in Supplementary Fig. S1). At an age of 12 weeks, pancreata were harvested and abnormal areas analyzed. A, Hematoxylin and eosin (H&E) staining of representative areas. Scale bar, 50 μm. B, The bar graph shows a quantification of the abnormal areas in control- or pomalidomide-treated KC mice (n = 4 per treatment group). *, statistical significance (P < 0.05) as compared with control. The pie graphs show grading of pancreatic lesions and relative presence of ADM, ADM-PanIN transition areas, and PanIN1. C and D, Trichrome staining or IHC staining for SMA of lesion areas in KC mice after control or pomalidomide treatment. Representative areas are shown. Scale bar, 100 μm in C and 50 μm in D. E, Quantification of SMA content of abnormal pancreatic areas. IHC-stained samples (n = 3) from each treatment group were analyzed. The white bars show the SMA content at treatment start as a reference (n = 3 samples). *, statistical significance (P < 0.05).
Ligia I. Bastea et al. Cancer Res 2019;79:
©2019 by American Association for Cancer Research