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N1 decoys that block JAG elevate sVEGFR1/sFLT1.
N1 decoys that block JAG elevate sVEGFR1/sFLT1. A, HEY1, HEY2, HEYL, and HES1 qRT-PCR of N1 decoys or JAG1 knockdown (KD) HUVECs. B–D, VEGF receptor qRT-PCR and flow cytometry of N1 decoys or JAG1 KD HUVECs. E, soluble VEGFR1 (sVEGFR1/sFLT1) qRT-PCR of N1 decoys or JAG1 KD HUVECs. A to E, data, as ± SD; *, P < F, soluble VEGFR1/sFLT1 ELISA presented as ± SD; *, P < G, VEGFR1 staining of Mm5MT-FGF4 tumor sections; scale bars, 30 μm. H, quantitation of the percentage of the mean VEGFR1–positive area; **, P < (n = 3). Thaned Kangsamaksin et al. Cancer Discovery 2015;5: ©2015 by American Association for Cancer Research
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