1. IDO deficiency is associated with attenuated induction of IL-6 during 4T1 tumor metastasis.
IDO deficiency is associated with attenuated induction of IL-6 during 4T1 tumor metastasis. A, evaluation of IDO1 protein levels by immunoprecipitation–Western blot analysis of lung tissue lysates from WT and Ido1−/− mice following orthotopic engraftment of 4T1 tumor cells at the time points in weeks indicated above each lane. C, epididymis lysate positive control lane; M, molecular weight marker lane. B, evaluation of kynurenine levels by LC/MS-MS–based analysis of homogenized lung samples from WT and Ido1−/− mice following orthotopic engraftment of 4T1 tumor cells at the time points in weeks indicted for each lane. Means ± SEM (n ≥ 6) are graphed with significance relative to baseline determined by one-way ANOVA with Dunn test (*, P < 0.05; **, P < 0.01). C, IL-6 level determinations from cytokine bead array immunoassay–based analysis of homogenized lung samples from WT and Ido1−/− mice following orthotopic engraftment of 4T1 tumor cells at the time points in weeks indicted for each lane. Means ± SEM (n ≥ 3) are graphed with significance relative to baseline determined by one-way ANOVA with Dunn test (*, P < 0.05; **, P < 0.01).
Courtney Smith et al. Cancer Discovery 2012;2:
©2012 by American Association for Cancer Research