1. Volume 7, Issue 6, Pages 1057-1060 (June 2014)
OsRFPH2-10, a RING-H2 Finger E3 Ubiquitin Ligase, Is Involved in Rice Antiviral Defense in the Early Stages of Rice dwarf virus Infection 
Liu Lifang , Jin Lian , Huang Xiahe , Geng Yongtao , Li Feng , Qin Qingqing , Wang Rui , Ji Shaoyi , Zhao Shanshan , Xie Q.i. , Wei Chunhong , Xie Can , Ding Biao , Li Y.i.  
Molecular Plant 
Volume 7, Issue 6, Pages (June 2014)
DOI: /mp/ssu007
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
OsRFPH2-10 (Referred to as H2-10) Promotes RDV P2 Degradation and Plays an Important Antiviral Function at Early Stages of RDV Infection.
(A) Yeast two-hybrid assay for AD-H2-10 and BD-P2 interaction.
(B) Co-IP analysis of FLAG-H2-10 and HA-P2 in planta.
(C) E3 ubiquitin ligase activity of H2-10. The numbers on the left denote the molecular masses of marker proteins in kilodaltons. The nickel-horseradish peroxidase was used to detect His-tagged ubiquitin (top panel) and the anti-MBP antibodies were used for detecting maltose fusion proteins (bottom panel).
(D) Effects of H2-10 on the stability of P2 in plant cells. Numbers indicate the ratios of agrobacteria concentrations used in co-infiltration.
(E) Effects of MG132 on the stability of P2 in plant cells.
(F) White specks on the leaves of RDV-infected wild-type (WT) and H2-10-overexpression rice plants. The arrowheads indicate the specks on the leaves.
(G) Detection of P2 and P8 accumulations in RDV-infected WT and H2-10 overexpression rice lines (H2-10 oe #1 and #5). H, mock-inoculated; R, RDV-infected.
(H) Detection of the accumulation of RDV RNAs S2, S8, and S11 in RDV-infected WT and H2-10-overexpression rice lines with corresponding 32P-labeled probes.
(I) RDV infection rates. wpi, week post inoculation. * P < 0.05; ** P < Inoculation assays were repeated three times, respectively. The error bars indicate standard errors.
Molecular Plant 2014 7, DOI: ( /mp/ssu007)
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions