1. UNC10112785 drives MYC protein loss.
UNC drives MYC protein loss. (A) Chemical structure of UNC The red circle indicates the position at which different analogs were synthesized. (B) GPS-MYC cells were treated with 20 μM UNC for 6 hours, and EGFP and DsRed intensity was measured by flow cytometry. Data from the GPS-MYC screen. (C) GPS-MYC cells were treated with UNC for 6 hours, and EGFP-MYC and MYC abundance was measured by immunoblotting. (D) PDAC cells were treated with UNC , and MYC protein abundance was measured by immunoblot. (E) Kinase selectivity of UNC as described previously (30). (F) MIA PaCa-2 cells were treated for 6 hours with the indicated compounds, and MYC protein abundance was measured by immunoblot. All data are representative of at least three independent experiments.
Devon R. Blake et al., Sci. Signal. 2019;12:eaav7259
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