1. Omomyc-induced cytoskeletal reorganization and increased apoptosis in C2C12 cells.
Omomyc-induced cytoskeletal reorganization and increased apoptosis in C2C12 cells. A, omomer expression (Western blot) in C2C12 cells harboring control (BP), Omomer, or Myc and Omomer retroviruses (Myc + Omomer). B, actin fiber formation. Myc-C2C12 myoblasts harboring empty or Omomer retroviruses (Myc and Myc + Omomer, respectively) were stained with FITC-phalloidin. C, potentiation of Myc apoptosis. Myc and Myc + Omomer C2C12 cells, serum deprived and incubated with tamoxifen, were stained with Hoechst dye. Some nuclei displaying apoptotic morphological features are indicated by arrows. D, FACS analysis of serum-deprived cells. The subdiploid fraction, characteristic of apoptosis, represented 15% of the population in Myc cells and amounted to 25% in Myc + Omomer cells.
Laura Soucek et al. Cancer Res 2002;62:
©2002 by American Association for Cancer Research