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Fig. 2 6-HAP does not inhibit DNA synthesis in vitro.

Published byΠανκρατιος Ανδρεάδης Modified over 5 years ago

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Presentation on theme: "Fig. 2 6-HAP does not inhibit DNA synthesis in vitro."— Presentation transcript:

1 Fig. 2 6-HAP does not inhibit DNA synthesis in vitro.
6-HAP does not inhibit DNA synthesis in vitro. (A) Primer-template design. (B) DNA polymerase primer extension reactions in the presence of 6-HAP or with control solvent, DMSO. 6-HAP (1 mM) did not prevent incorporation of dATP opposite template T, even when in 100- to 10,000-fold excess. The reaction was with the Exo− Klenow fragment of E. coli Pol I, 0.25 U per reaction, for 10 min at 37°C. See Materials and Methods for a full description and fig. S1 for additional corroborating results. Stanislav G. Kozmin et al. Sci Adv 2019;5:eaaw3915 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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