1. Validation of OMV- and OM-enriched proteins.
Validation of OMV- and OM-enriched proteins. Candidate ORFs that encode proteins identified in our MS analysis as OMV or OM enriched were cloned into pFD340 with a C-terminal 6×His tag. Constructs were introduced into B. thetaiotaomicron by conjugation, generated strains were grown in TYG medium, and fractions were prepared. Ten micrograms of each fraction was subjected to 12% SDS-PAGE and analyzed by Western blotting using anti-His polyclonal antibodies. (a) OMV-enriched proteins; (b) OM-enriched proteins. The isoelectric point as well as the residues following the lipoprotein attachment cysteine (for lipoproteins) are indicated below the protein name.
Ezequiel Valguarnera et al. mSphere 2018; doi: /mSphere