1. PI3Kγ promotes macrophage PDGF-BB expression to control PDAC fibrosis.
PI3Kγ promotes macrophage PDGF-BB expression to control PDAC fibrosis. A, Masson’s Trichrome staining of tissue sections of pancreata from WT and p110γ−/− KC and KPC animals. Scale bar, 100 μm. B, Masson’s Trichrome staining of sections of pancreata from control- and TG –treated KPC animals. C and D, images (C) and quantification (D) of picrosirius red staining of sections of pancreata from KPC tumors grown in WT and p110γ−/− animals. Scale bar, 100 μm. E, collagen I immunostaining of LMP tumors from animals treated with the PI3Kγ inhibitor TG or chemically similar inert control. Scale bar, 100 μm. F, Western blot and quantification of collagen I protein expression in LMP tumors from animals treated with the PI3Kγ inhibitor TG or chemically similar inert control. G, relative collagen I mRNA expression in normal pancreata and LMP tumors from animals treated with the PI3Kγ inhibitor TG or chemically similar inert control. H, relative collagen I mRNA expression in primary murine fibroblasts incubated in the presence or absence of stimulus-free CM from IL4-stimulated WT and p110γ−/− macrophages. I, relative collagen I mRNA expression in fibroblasts incubated in the presence or absence of stimulus-free CM from IL4-stimulated WT macrophages in the absence or presence of anti-TGFβ, anti–PDGF-BB, or imatinib. Significance testing was performed by parametric Student t test.
Megan M. Kaneda et al. Cancer Discov 2016;6:
©2016 by American Association for Cancer Research