1. CLASSIFICATION OF ANAEMIA By GEORGE

2. CLASSIFICATION OF ANAEMIA

3. Anaemia is mainly based on the cause but it can also be classified on the basis of Red blood cells morphology on a stained blood or bone marrow smear. classification due to cause of Anaemia 1. Blood loss or Hemorrhagic anaemia This is the anaemia resulting from either acute massive blood loss or chronic blood loss. ie acute massive blood loss including accidents, antepartum and postpartum hemorrhage. Chronic blood loss can be due to GIT lesions, cancers, hypermenorrhea, parasitic infestation e.g hookworm infection. Blood transfusion is a reliable means of rapidly restoring blood volume.

4. Laboratory diagnosis  Hb is low  PCV is low  MCV, MCH, MCHC are usually normal Microscopic examination  RBCs.Normocytic normochromic.Polychromasia.Erythroblasts  WBCs.Normal in number and morphology.There can be neutrophil leucocytosis with toxic granulation  Platelets.usually normal but as time goes on thrombocytosis exists

5. Cont… 2.Haemolytic Anaemia This is anaemia due to excessive destruction and breakdown (haemolysis) of Red blood cells either extra vascularly (RBCs are destroyed by macrophages in the spleen, liver and bone marrow.) or intra vascularly (RBCs are destroyed in blood vessels e.g. malaria, incompatible BT, toxic chemicals and drugs). After 120 days RBCs are removed from circulation by the cells of RES. But the important feature of haemolysis is the shortened lifespan of the individual RBCs. i. Hereditary haemolytic Anaemia Here the abnormality is passed from the parents to the child.eg a. sickle cell disease It is an hereditary disease in which the RBCs of a person contain abnormal Hb. Here the amino acid Valine replaces the Glutamic acid of a normal HbA at partition 6 of the globlin molecule.

6. Laboratory diagnosis Sickle cell disease It is the homozygous state of the a disease HbSS  Hb is low  ESR is low  PCV is low  MCV, MCH, MCHC are normal although MCV may be higher because of polychromatic red cells. (CBC in 12 months old child) HBMCVWBCPlatelets Normal (AA)Normal HbASNormal HbSS6 – 9g/dLNormalNormal or Increased

7. Microscopic examination  RBCs.Marked Polychromasia.Sickled RBCs.Erythroblasts.Target cells.Howell jolly bodies  WBCs.Normal or Increased in number  Platelets.Normal

8. Sickled RBCs Normal RBCs

9. Hereditary haemolytic Anaemia cont… b. Hereditary Spherocytosis RBCs are more spherical and lack the central area of pallor on a stained blood film

10. Laboratory diagnosis  Hb is low  PCV is low  MCHC and MCH are high  MCV is low Microscopic examination  RBCs.Spherocytes.Polychromasia  WBCs.Normal  Platelets. Normal

11. Hereditary haemolytic Anaemia cont… c. Hereditary Elliptocytosis Is a membrane defect where the RBCs are oval or elliptical in shape

12. Hereditary haemolytic Anaemia cont… d. Paroxysmal Nocturnal Haemoglobinuria Is a rare acquired life threatening disease of blood characterized by destruction of RBCs by the complement system. Laboratory diagnosis  Hb is low  PCV is low  MCH, MCHC and MCV may be decreased Microscopic examination  RBCs.Marked Polychromasia.Microcytic cells  WBCs.Abnormal Morphology and distribution  Platelets.Thrombocytopenia

13. Paroxysmal Nocturnal Haemoglobinuria

14. ii. Acquired Haemolytic Anaemia Here the abnormality is acquired during life, the person is born normal.eg i. Haemolytic Disease of the Newborn (HDN) It’s due to ABO and rhesus incompatibility between the mother and the fetus. ii. incompatible blood transfusion This is due to ABO mismatch between the donor blood and patients serum. Laboratory diagnosis  Hb is low  PCV is low  MCV is high  MCHC and MCH are normal

15. Microscopic examination  RBCs.Nucleated.Polychromasia.Schistocytes  WBCs.Usually Normal  Platelets.Normal in number and morphology iii. Auto Immune Haemolytic Anaemia Here the person develops an antibody against own red cells

16. iv. Plasmodium parasite infection

17. Laboratory diagnosis  Hb is low  PCV is low  MCV, MCH and MCHC maybe normal Microscopic examination  RBCs.infected with plasmodium  WBCs.Maybe normal in low parasiteamia  Platelets.Decreased

18. 3.Deficiency Anaemia(Nutritional) This is anaemia caused by lack or deficiency of one or more substances necessary for Hb synthesis. eg Iron and materials necessary for growth of blood cells. eg Vitamin B12 or Folic Acid. a. Iron deficiency Anaemia It’s lack of iron in the body due to;  Impaired absorption of iron in the GIT  Insufficient iron in the diet  Hookworm infestation  Depletion of body iron stores Laboratory diagnosis  Hb is low  PCV is low  MCH,MCV and MCHC will be low  RDW is raised

19. Microscopic examination  RBCs.Marked Hypochromasia.Microcytosis.Target cells.Poikilocytes.Pencil shaped cells.Tear drop cells  WBCs.Normal in number and morphology but it increases and in case of hookworm infestation, eosinophilia is seen.  Platelets.Normal in number and morphology

20. Iron deficiency Anaemia cont…  Biochemical tests

21. Iron deficiency Anaemia cont…

22. 3.Deficiency Anaemia(Nutritional) b. Vitamin B12/Folic acid deficiency Anaemia It’s lack of Vitamin B12 or Folic acid due to;  Impaired absorption in the GIT  Insufficient Vitamin B12 and Folic acid in the Diet  Worm infestation especially D.latum  Lack of gastric intrinsic factor and HCL in the stomuch Laboratory diagnosis  Hb is low  PCV is low  MCV is high  MCH and MCHC are Normal

23. Microscopic examination  RBCs.Anisocytosis.Macrocytes.Ovalocytes.Howell jolly bodies  WBCs.Neutrophils with hypersegmented nuclear lobes are common  Giant Platelets are common

24. classification due to cause of Anaemia cont… 4. Aplastic anaemia Is anaemia due to replacement of haemopoietic tissues of the red bone marrow by fibrous non haemopoietic tissues. Causes include; Certain drugs Irradiation Diseases such as Viral Hepatitis Bone marrow invasion by abnormal cells such as leukemia Laboratory diagnosis  Hb low (sometimes normal depending on the extent of marrow aplasia)  PCV maybe normal  MCH, MCH and MCHC may also be normal

25. Aplastic anaemia cont… Microscopic examination  RBCs.Normochromic normocytic.Slight Macrocytes.Decreased Reticulocytes count (less than 1%)  WBCs.Leucopenia  Platelets.Thrombocytopenia

26. Aplastic anaemia cont… Nomal BMS Aplastic Anaemia BMS

27. classification due to cause of Anaemia cont… 5. Anaemia of Chronic Diseases This arises due to some chronic illness such as TB, Leukemia, Multiple Myeloma (Myelomatosis) AML

28. Morphological classification of Anaemia 1. Normocytic Normochromic Anaemia Here the cells stain normally, ie normal colour and shape. Conditions include;.Blood loss Anaemia.Aplastic Anaemia Laboratory diagnosis  Hb ≤ 12 g/dL  MCV 80 – 100  MCHC > 30 Microscopic examination  RBCs.Appear normal

29. Morphological classification of Anaemia cont… 2. Hypochromic Microcytic Here RBCs are under stained due to loss of Hb and majority are smaller in size than normal. Conditions include;.Iron deficiency anaemia.sideroblastic anaemia. Thalassemias.Anaemia of chronic disease Laboratory diagnosis  MCV is low (< 80)  MCHC is low (< 31)

30. Hypochromic Microcytic cont… Microscopic examination  RBCs.Anisocytosis.Anisochromasia ( marked variability in colour density of erythrocytes which indicates unequal hemoglobin content of RBCs ).Elliptocytosis.Basophilic stippling.Target cells.Tear drop cells

31. classification due to cause of Anaemia cont… 3. Normochromic Macrocytic Anaemia Here the RBCs stain normally (pink) but majority are larger in size than normal. Conditions include;.Vitamin B12/ Folic Acid deficiency Anaemia.Some Haemolytic Anaemias.Liver disease. Laboratory diagnosis  MCV is high > 100  MCHC is always normal

32. Normochromic Macrocytic Anaemia cont… Microscopic examination  RBCs.Ovalocytes.Macrocytes.Howell jolly bodies.Anisocytes.Poikilocytes

33. CLASSIFICATION OF ANAEMIA END Thanks