1. CHL-induced apoptosis in HCT116 cells involves the activation of procaspase-8 and procaspase-6, with cleavage of nuclear lamins.
CHL-induced apoptosis in HCT116 cells involves the activation of procaspase-8 and procaspase-6, with cleavage of nuclear lamins. A, Western blot showing the presence of cleaved caspase-8 (active form) at all concentrations of CHL. NS, nonspecific band (putative cleavage product). B, immunoblot showing the presence of cleaved caspase-6 at higher concentrations of CHL. C, concentration-dependent cleavage of lamin A by CHL and the presence of cleaved lamin A fragments. D, ethidium bromide-stained agarose gel showing a lack of oligonucleosomal DNA fragmentation in the floating cell population collected after treatment with CHL. The positive control for DNA laddering was 4 mm sodium butyrate (far right lane). Results in A−D are representative of data obtained from three or more separate experiments, 24 h after cells had been treated with CHL (or butyrate).
G. Darío Díaz et al. Cancer Res 2003;63:
©2003 by American Association for Cancer Research