1. A, cellular uptake of AS1411 in MCF-7 and MCF-10A cells.
A, cellular uptake of AS1411 in MCF-7 and MCF-10A cells. The binding of AS1411 was determined by confocal imaging of immunofluorescence of FITC labeled AS1411. Nuclei were counterstained with propidium iodide (PI). Representative images of three separate experiments. B, levels of nucleolin and bcl-2 protein in MCF-7 and MCF-10A cells. The levels of nucleolin and bcl-2 protein were determined by immunoblotting with mAbs specific for nucleolin and bcl-2 proteins. The relative amounts of protein were calculated after normalization to β-actin for the cytosolic extracts and to histone 2B for nuclear extracts.
Sridharan Soundararajan et al. Cancer Res 2008;68:
©2008 by American Association for Cancer Research