1. A, TSG-6 staining in human prostate.
A, TSG-6 staining in human prostate. Human prostate tissue array containing different grade tumors with paired normal prostate was stained with TSG-6. TSG-6 was used at a dilution of 1:100 in PBS and incubated overnight at 4°C. Immune complexes were revealed using a universal secondary antibody (100 μL for 30 minutes) followed by chromogen as described in Materials and Methods. Left, little or no TSG-6 staining in the cancerous tissue; right, TSG-6 expression in normal prostate (P = ). Statistical significance of the data was determined using ANOVA and bootstrap methods as described in Materials and Methods. B, variability of TSG-6 expression in human prostate tumors. Tissue array containing human prostate tumors from different Gleason grade were incubated with a protein blocking serum-free reagent for 60 minutes to block nonspecific binding. TSG-6 was used at a dilution of 1:100 in PBS and incubated overnight at 4°C. Immune complexes were revealed using a universal secondary antibody (100 μL for 30 minutes) followed by chromogen. Negative controls were included by omitting the primary antibody (data not shown).
Gretchen E. Garcia et al. Clin Cancer Res 2006;12:
©2006 by American Association for Cancer Research