1. Notch inhibits apoptosis by direct interference with XIAP ubiquitination and degradation
Interaction between XIAP and the endogenously generated Notch intracellular domain. (A) Endogenously generated NICD stabilized XIAP. DO11.10 cells were stimulated with immobilized Jagged‐1‐Fc and total cell lysates prepared at 1, 2, 4, and 6 h after stimulation. The Notch1 intracellular domain generated was detected by antibody specific for the cleaved Notch1 intracellular domain (Val1744), whereas the full‐length Notch1 was examined by anti‐Notch1 extracellular domain (G‐20). The amounts of NICD and XIAP were quantitated by densitometry, normalized against GAPDH contents, and plotted versus time of stimulation. The number is the average of two experiments. (B) Association of the endogenous NICD with XIAP. Total cell lysates from (A) were immunoprecipitated using anti‐XIAP antibodies and the immune complexes analyzed by Western blot with Val1744 and G‐20 antibodies. Immunoprecipitation was also performed with Val1744 antibodies and protein complexes assayed with anti‐XIAP antibody.
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EMBO J, Volume: 26, Issue: 6, Pages: , First published: 22 February 2007, DOI: ( /sj.emboj )