1. Volume 3, Issue 6, Pages 1049-1063 (November 2010)
Functional Characterization and RNAi-Mediated Suppression Reveals Roles for Hexose Transporters in Sugar Accumulation by Tomato Fruit 
McCurdy David W. , Dibley Stephen , Cahyanegara Ricky , Martin Antony , Patrick John W.  
Molecular Plant 
Volume 3, Issue 6, Pages (November 2010)
DOI: /mp/ssq050
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

2. Figure 1 Phylogenetic Analysis of LeHTs.
LeHT sequences are indicated by arrows. The tree was constructed using Phylogeny.fr (Dereeper et al., 2008) as described in Methods. Database accession numbers of the sequences used are: S. lycopersicum LeHT1 (GQ911580), LeHT2 (CAB52689), LeHT3 (GQ911581); A. thaliana AtSTP1 (NP_172592), AtSTP2 (AJ001362), AtSTP3 (CAA05384), AtSTP4 (BAB01309), AtSTP5 (NM_103182), AtSTP6 (AJ001659), AtSTP7 (CAB80698), AtSTP8 (AF077407), AtSTP9 (AJ001662), AtSTP10 (DQ056599), AtSTP11 (AJ001664), AtSTP12 (NP_193879), AtSTP13 (CAC69074), AtVGT1 (NP_186959), AtTMT1 (Z50752), AtTMT2 (AJ532570), AtTMT3 (AJ532571); V. vinifera VvHT1 (AJ001061), VvHT2 (AY663846), VvHT6 (AY861386), VvHT7 (AY854146), pGLT (AY608701), VvHT3 (AY538259), VvHT4 (AY538260), VvHT5 (AY538261); O. sativa OsMST1 (AB052883), OsMST2 (AB052884), OsMST3 (AB052885), OsMST4 (AY342321), OsMST5 (NM_ ), OsMST6 (AY342322); L. polyphyllus LpSTP1 (Szenthe et al., 2007); A. comosus AcMST1 (EF460876); H. vulgare HvSTP1 (CAD58958).
Molecular Plant 2010 3, DOI: ( /mp/ssq050)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

3. Figure 2
Time Course and pH-Dependence of Hexose Transport by LeHT1 and LeHT2 Expressed in Yeast.
Time course of (A) [14C]glucose and (B) [14C]fructose uptake from media containing 50 μM sugars buffered at pH 5. pH-dependent uptake rates of (C) [14C]glucose and (D) [14C]fructose from HEPES/MES-buffered media containing 50 μM glucose or 800 μM fructose, respectively. Bars are standard errors of means derived from a minimum of four replicates per treatment.
Molecular Plant 2010 3, DOI: ( /mp/ssq050)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

4. Figure 3
Concentration-Dependent Uptake of Hexoses by LeHT1 and LeHT2 Expressed in Yeast.
Concentration-dependent uptake of fructose (A–D) and glucose (E) facilitated by LeHT1 (A, C, E) or LeHT2 (B, D) from media buffered at pH 5 (A, B, E) or pH 6 (C, D). Bars are standard errors of means derived from a minimum of four replicates per treatment. Apparent Km and Vmax values were derived by fitting non-linear regressions of the Michaelis-Menten rate equation to the data using least-squares. The degree of fit is indicated by R2 values.
Molecular Plant 2010 3, DOI: ( /mp/ssq050)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions