1. Expression of Rapgef2 and Rapgef6 in the E13.5 and E15.5 brains.
Expression of Rapgef2 and Rapgef6 in the E13.5 and E15.5 brains. A, Immunohistological detection of Rapgef2 and Rapgef6. Coronal sections of the brains of Rapgef2-f/f (2-f/f), Rapgef2-cKO (2-cKO), and Rapgef6-KO (6-KO) embryos at E13.5 and E15.5 were subjected to immunostaining for Rapgef2 or Rapgef6 (red) and 4',6-diamidino-2-phenylindole (DAPI; blue). Scale bars, 100 µm. B, Preferential expression of Rapgef2 and Rapgef6 on the apical surfaces. Coronal sections of the brains of the E13.5 and E15.5 embryos with the indicated genotypes were subjected to immunostaining for Rapgef2 or Rapgef6 (green), β-catenin (red), and DAPI (blue). The images are representative of four biological replicates of each group. Scale bars, 20 µm.
Kazuhiro Maeta et al. eneuro 2016;3:ENEURO
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